DAO 119:173-177 (2016) - DOI: https://doi.org/10.3354/dao02987
NOTE
ORF13 in the Type III secretion system gene cluster of Edwardsiella tarda binds to the mammalian factor Cugbp2
Jun Okuda1,2,*, Yusuke Takeuchi1, Masashi Yasuda2, Toshihiro Nakai1
ABSTRACT: The Type III secretion system (TTSS) is essential for the intracellular replication of Edwardsiella tarda in phagocytes of fish and mammals, and a hypothetical gene (orf13) located in the TTSS gene cluster is required for intracellular replication and virulence of E. tarda. Here, we show that under TTSS-inducing conditions, the protein ORF13 was secreted into culture supernatant. Then, using a yeast 2-hybrid screen, we show that the mammalian factor Cugbp2, which regulates apoptosis in breast cancer cells, directly interacts with ORF13. A pull-down assay revealed that ORF13 binds to the C-terminal region of Cugbp2. Our results suggest that ORF13 may facilitate E. tarda replication in phagocytes by binding to Cugbp2.
KEY WORDS: Edwardsiella tarda · Type III secretion system · ORF13 · Mammalian factor Cugbp2
| Full text in pdf format | Cite this article as: Okuda J, Takeuchi Y, Yasuda M, Nakai T
(2016) ORF13 in the Type III secretion system gene cluster of Edwardsiella tarda binds to the mammalian factor Cugbp2. Dis Aquat Org 119:173-177. https://doi.org/10.3354/dao02987
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