Diseases of Aquatic Organisms
DAO is a hybrid research journal on all aspects of disease phenomena in aquatic organisms.
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DOI: https://doi.org/10.3354/dao03023
copiedIsolation and genetic characterization of Nocardia seriolae from snubnose pompano Trachinotus blochii in Vietnam
ABSTRACT: A total of 480 cage-cultured fish were collected from 4 coastal provinces in central Vietnam to investigate the causative agent of nocardiosis. Fish displayed unique characteristics such as paleness and lethargy and exhibited haemorrhages and ulcers on the skin. Prominent white nodules varying in size were observed in the spleen, kidney, and liver. Furthermore, histopathological sections showed typical granulomatous lesions in these organs. Using the Ziehl-Neelsen staining method, isolated bacteria exhibited acid-fast, bead-like filament morphology when cultured in brain-heart infusion medium or Ogawa medium. Phylogenetic analysis of 16S rDNA confirmed that the isolated bacterium was Nocardia seriolae. This study demonstrates for the first time an outbreak of N. seriolae in snubnose pompano in central Vietnam.
KEYWORDS
Hung Vu-Khac (Corresponding Author)
- Department of Biotechnology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
Van Quy Binh Duong (Co-author)
- Department of Biotechnology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
S.-F. Chen (Co-author)
- Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Ping Tung, 912, Taiwan
Trung Hieu Pham (Co-author)
- Department of Biotechnology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
Thi Thu Giang Nguyen (Co-author)
- Department of Biotechnology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam
Thi Thu Hang Trinh (Co-author)
- Department of Biotechnology, Central Vietnam Veterinary Institute, km4 Dong De Street, Nha Trang, Vietnam