Inter-Research > DAO > v120 > n3 > p231-240  
Diseases of Aquatic Organisms

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DAO 120:231-240 (2016)  -  DOI:

Ostreid herpesvirus in wild oysters from the Huelva coast (SW Spain)

M. López-Sanmartín1,*, J. R. López-Fernández1, M. E. Cunha2, R. De la Herrán3, J. I. Navas1

1IFAPA Centro Agua del Pino, Consejería de Agricultura, Pesca y Desarrollo Rural, Junta de Andalucía, Ctra. El Rompido-Punta Umbría Km 3.8, 21459 Cartaya, Huelva, Spain
2Estação Piloto de Piscicultura de Olhão, Instituto Português do Mar e Atmosfera, Av. 5 de Outubro, s/n, 8700-305 Olhão, Portugal
3Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain
*Corresponding author:

ABSTRACT: This is the first report of ostreid herpesvirus 1 microvariant (OsHV-1 µVar) infecting natural oyster beds located in Huelva (SW Spain). The virus was detected in 3 oyster species present in the intertidal zone: Crassostrea gigas (Thunberg, 1793), C. angulata (Lamarck, 1819) and, for the first time, in Ostrea stentina Payraudeau, 1826. Oysters were identified by a specific polymerase chain reaction (PCR) and posterior restriction fragment length polymorphism (RFLP) analysis based on cytochrome oxidase I (COI) mitochondrial DNA. Results confirmed that C. angulata still remains the dominant oyster population in SW Spain despite the introduction of C. gigas for cultivation in the late 1970s, and its subsequent naturalization. C. angulata shows a higher haplotype diversity than C. gigas. OsHV-1 virus was detected by PCR with C2/C6 pair primers. Posterior RFLP analyses with the restriction enzyme MfeI were done in order to reveal the OsHV-1 µVar. Detections were confirmed by DNA sequencing, and infections were evidenced by in situ hybridization in C. gigas, C. angulata and O. stentina samples. The prevalence was similar among the 3 oyster species but varied between sampling locations, being higher in areas with greater harvesting activities. OsHV-1 µVar accounted for 93% of all OsHV-1 detected.

KEY WORDS: Crassostrea gigas · Crassostrea angulata · Ostrea stentina · Herpesvirus · Microvariant · OsHV-1 µVar · In situ hybridization

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Cite this article as: López-Sanmartín M, López-Fernández JR, Cunha ME, De la Herrán R, Navas JI (2016) Ostreid herpesvirus in wild oysters from the Huelva coast (SW Spain). Dis Aquat Org 120:231-240.

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