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Diseases of Aquatic Organisms

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DAO 126:51-62 (2017)  -  DOI:

Importation of CyHV-2-infected goldfish into the Netherlands

Takafumi Ito1,*, Jun Kurita1, Olga L. M. Haenen2

1Tamaki Laboratory, Research Center for Fish Diseases, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, 224-1 Hiruda, Tamaki, Mie 519-0423, Japan
2National Reference Laboratory for Fish Diseases, Wageningen Bioveterinary Research, Wageningen University & Research, PO Box 65, 8200 AB Lelystad, The Netherlands
*Corresponding author:

ABSTRACT: Cyprinid herpesvirus 2 (CyHV-2) is known as the causative agent of herpesviral haematopoietic necrosis in goldfish Carassius auratus auratus. However, the virus has also been detected in Prussian carp C. gibelio and crucian carp C. carassius from European and Asian countries. To prevent spread of the causative virus to other areas, investigation of the risk factors of spread of this virus is important. In this study, 8 batches of goldfish imported into the Netherlands by airfreight from Asia and the Middle East were investigated for the presence of the virus. CyHV-2 DNA was detected by PCR in the pooled kidneys of 4 of the 8 imported goldfish batches, of which 1 was from a CyHV-2 disease case at a Dutch importer’s quarantine facility. Sequence analysis of the CyHV-2 strains from this study and from previous reports showed that there were at least 6 different lengths in the mA region, resulting in tentatively at least 4 genotypes. Virus isolation was positive for only 1 (Amsterdam Schiphol-1 [AMS-1]) of the 8 samples. It was shown that the AMS-1 isolate was highly virulent to Ryukin goldfish after 100.3 TCID50 fish-1 intraperitoneal injection. The viral titre of the AMS-1 isolate for goldfish fin cells at several temperatures was similar to that of a Japanese CyHV-2 isolate. Our results prove that one of the routes of spread of various CyHV-2 strains is through the global trade of apparently healthy infected goldfish.

KEY WORDS: Cyprinid herpesvirus 2 · Goldfish · Ornamental fish · International trade · Disease spread · Risk factor · Genotype

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Cite this article as: Ito T, Kurita J, Haenen OLM (2017) Importation of CyHV-2-infected goldfish into the Netherlands. Dis Aquat Org 126:51-62.

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