DAO 127:243-247 (2018)  -  DOI: https://doi.org/10.3354/dao03203

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Systematic approach to isolating Batrachochytrium dendrobatidis

Anthony W. Waddle*, Marlai Sai, Joshua E. Levy, Ghazal Rezaei, Frank van Breukelen, Jef R. Jaeger

School of Life Sciences, University of Nevada, Las Vegas, 4505 S. Maryland Parkway, Las Vegas, NV 89154, USA
*Corresponding author:

ABSTRACT: We developed a protocol for isolating the amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) from anurans. We sampled skin tissues from 2 common treefrogs, Pseudacris regilla and P. triseriata, collected from populations with high infection prevalence. We sampled tissues from 3 anatomical ventral regions (thigh, abdomen, and foot) where the pathogen is thought to concentrate. To mitigate potential bacterial contamination, we used a unique combination of 4 antibiotics. We quantified infections on frogs as zoospore equivalents (ZE) using a swabbing approach combined with quantitative real-time polymerase chain reaction. We isolated Bd from 68.9% of frogs sampled from both species. Contamination was low (9.7% of all plates), with most contamination presumed to be fungal. We found positive correlations between successful isolation attempts and infection intensity. Our levels of isolation success were 74% for P. triseriata and 100% for P. regilla once Bd detection intensities reached ≥40 ZE. Of the 3 anatomical regions sampled in both species, we had significantly more success isolating Bd from foot tissue. Our results support published recommendations to focus sampling for Bd infection on feet, particularly webbing.


KEY WORDS: Amphibian · Chytridiomycosis · Fungus · Isolation · Protocol


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Cite this article as: Waddle AW, Sai M, Levy JE, Rezaei G, van Breukelen F, Jaeger JR (2018) Systematic approach to isolating Batrachochytrium dendrobatidis. Dis Aquat Org 127:243-247. https://doi.org/10.3354/dao03203

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