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Diseases of Aquatic Organisms

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DAO 128:81-86 (2018)  -  DOI: https://doi.org/10.3354/dao03212

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Host range and influence of a cell capsule on the phage efficacy of three Lactococcus garvieae lytic phages

Truong Dinh Hoai1,2, Issei Nishiki3,*, Terutoyo Yoshida1, Toshihiro Nakai4

1Faculty of Agriculture, University of Miyazaki, Gakuen kibanadai nishi 1-1, Miyazaki 889-2192, Japan
2Faculty of Fisheries, Vietnam National University of Agriculture, Ngo Xuan Quang, Trau Quy, Gia Lam, Hanoi 131004, Vietnam
3Research Center for Bioinformatics and Biosciences, National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, 2-14-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan
4Laboratory of Fish Pathology, Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima 739-8528, Japan
*Corresponding author:

ABSTRACT: Three lytic phages (PLgW-1, PLgY-16, and PLgY-30) were previously used for phage-typing Lactococcus garvieae, a bacterial pathogen of various marine fish species, and were demonstrated to be potential therapeutants for infections caused by L. garvieae. The morphology, host range, and efficacy of these phages have not been investigated in detail, however. The current study examined the lysis spectrum of these 3 phages against 16 different genotypes of L. garvieae and the influence of a bacterial capsule on phage efficacy, to aid in developing an effective treatment for lactococcosis in fish. Morphological analysis by transmission electron microscopy revealed that all 3 phages belonged to the family Siphoviridae and had a minor difference in morphology. These phages lysed a high proportion of their bacterial host (93.7% of the different L. garvieae genotypes). In addition, the efficacy of the plating assays was affected by both the phages and their bacterial host, in which phage efficacy was clearly affected by a bacterial capsule. The results of this study may be useful for developing appropriate strategies to use these phages to control various genotypes of L. garvieae causing disease in marine fish.


KEY WORDS: Lytic phage · Host range · Cell capsule · Efficacy · Lactococcus garvieae · Marine fish


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Cite this article as: Hoai TD, Nishiki I, Yoshida T, Nakai T (2018) Host range and influence of a cell capsule on the phage efficacy of three Lactococcus garvieae lytic phages. Dis Aquat Org 128:81-86. https://doi.org/10.3354/dao03212

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