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Diseases of Aquatic Organisms

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DAO 128:127-145 (2018)  -  DOI: https://doi.org/10.3354/dao03220

Proteomic profile of Ostrea edulis haemolymph in response to bonamiosis and identification of candidate proteins as resistance markers

Nuria R. de la Ballina1, Antonio Villalba1,2,*, Asunción Cao1

1Centro de Investigacións Mariñas (CIMA), Consellería do Mar, Xunta de Galicia, 36620 Vilanova de Arousa, Spain
2Departamento de Ciencias de la Vida, Universidad de Alcalá, 28871 Alcalá de Henares, Spain
*Corresponding author:

ABSTRACT: European flat oyster Ostrea edulis populations have suffered extensive mortalities caused by bonamiosis. The protozoan parasite Bonamia ostreae is largely responsible for this disease in Europe, while its congener B. exitiosa has been detected more recently in various European countries. Both of these intracellular parasites are able to survive and proliferate within haemocytes, the main cellular effectors of the immune system in molluscs. Two-dimensional electrophoresis was used to compare the haemolymph protein profile between Bonamia spp.-infected and non-infected oysters within 3 different stocks, a Galician stock of oysters selected for resistance against bonamiosis, a non-selected Galician stock and a selected Irish stock. Thirty-four proteins with a presumably relevant role in the oyster-Bonamia spp. interaction were identified; they were involved in major metabolic pathways, such as energy production, respiratory chain, oxidative stress, signal transduction, transcription, translation, protein degradation and cell defence. Furthermore, the haemolymph proteomic profiles of the non-infected oysters of the 2 Galician stocks were compared. As a result, 7 proteins representative of the non-infected Galician oysters selected for resistance against bonamiosis were identified; these 7 proteins could be considered as candidate markers of resistance to bonamiosis, which should be further assessed.


KEY WORDS: Bonamia ostreae · Bonamia exitiosa · Two-dimensional electrophoresis · Proteomics · Immune response · Selective breeding


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Cite this article as: de la Ballina NR, Villalba A, Cao A (2018) Proteomic profile of Ostrea edulis haemolymph in response to bonamiosis and identification of candidate proteins as resistance markers. Dis Aquat Org 128:127-145. https://doi.org/10.3354/dao03220

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