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Diseases of Aquatic Organisms

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DAO 128:175-185 (2018)  -  DOI: https://doi.org/10.3354/dao03228

An alloherpesvirus infection of European perch Perca fluviatilis in Finland

Kyle A. Garver1,*, Katja Leskisenoja2, Robert Macrae3, Laura M. Hawley1, Kuttichantran Subramaniam4, Thomas B. Waltzek4, Jon Richard1, Caroline Josefsson3, E. Tellervo Valtonen

1Fisheries and Oceans Canada, Pacific Biological Station, Nanaimo, British Columbia V9T 6N7, Canada
2Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä 40014, Finland
3Biology Department, Vancouver Island University, Nanaimo, British Columbia V9R 5S5, Canada
4Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida 32611, USA
*Corresponding author:
Deceased

ABSTRACT: The order Herpesvirales includes viruses that infect aquatic and terrestrial vertebrates and several aquatic invertebrates (i.e. mollusks), and share the commonality of possessing a double-stranded DNA core surrounded by an icosahedral capsid. Herpesviruses of the family Alloherpesviridae that infect fish and amphibians, including channel catfish virus and koi herpesvirus, negatively impact aquaculture. Here, we describe a novel herpesvirus infection of wild European perch from lakes in Finland. Infected fish exhibited white nodules on the skin and fins, typically in the spring when prevalence reached nearly 40% in one of the sampled lakes. Transmission electron microscopic examination of affected tissues revealed abundant nuclear and cytoplasmic virus particles displaying herpesvirus morphology. Degenerate PCR targeting a conserved region of the DNA polymerase gene of large DNA viruses amplified a 520 bp product in 5 of 5 affected perch skin samples tested. Phylogenetic analysis of concatenated partial DNA polymerase and terminase (exon 2) gene sequences produced a well-supported tree grouping the European perch herpesvirus with alloherpesviruses infecting acipenserid, esocid, ictalurid, and salmonid fishes. The phenetic analysis of the European perch herpesvirus partial DNA polymerase and terminase nucleotide gene sequences ranged from 34.6 to 63.9% and 39.6 to 59.6% to other alloherpesviruses, respectively. These data support the European perch herpesvirus as a new alloherpesvirus, and we propose the formal species designation of Percid herpesvirus 2 (PeHV2) to be considered for approval by the International Committee on Taxonomy of Viruses.


KEY WORDS: Herpesvirus · European perch · White nodules · Alloherpesvirus


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Cite this article as: Garver KA, Leskisenoja K, Macrae R, Hawley LM and others (2018) An alloherpesvirus infection of European perch Perca fluviatilis in Finland. Dis Aquat Org 128:175-185. https://doi.org/10.3354/dao03228

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