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Diseases of Aquatic Organisms

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DAO 128:187-201 (2018)  -  DOI: https://doi.org/10.3354/dao03231

Comparative susceptibilities and immune reactions of wild and cultured populations of Caspian trout Salmo trutta caspius to VHSV

Asma Mohammad Karami1,2, Ali Bani1,3,*, Mohammad Pourkazemi4, Mohades Ghasemi5, Per Walter Kania2, Kurt Buchmann2,*

1Department of Biology, Faculty of Science, University of Guilan, Rasht 4199613776, Iran
2Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C 1870, Denmark
3Department of Marine Science, Caspian Sea Basin Research Center, University of Guilan, Rasht 4199613776, Iran
4Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Tehran 14965-149, Iran
5Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organisation, Bandar Anzali 4316713111, Iran
*Corresponding authors: ,

ABSTRACT: Caspian trout Salmo trutta caspius is an endangered subspecies of brown trout Salmo trutta which is native to the Caspian Sea. Restocking programmes have been established, but recent introduction of the rhabdovirus viral haemorrhagic septicaemia virus (VHSV) into Iranian rainbow trout farms connected to waterbodies supporting wild Caspian trout may represent an additional threat to the declining stock. The susceptibility of wild and cultured populations of this endemic subspecies was demonstrated by performing controlled VHSV infection experiments (both by bath and injection challenges). Subsequently, VHSV infection in exposed fish was confirmed (CPE and quantitative PCR), virus levels were measured, and regulation of immune genes in exposed fish was investigated with a focus on the genes encoding IL-8, IFNγ, TGFβ, TNFα, SAA, C3-4, CD8α, IgM, MHC I, MHC II, iNOS and IGF-1. The presence of IgM-, CD8α- and MHC II-positive cells in host organs was visualized by immunohistochemistry. Both wild and cultured trout strains proved to be VHSV-susceptible following experimental challenge, but the mortality curves and associated regulation of immune-related genes differed between the 2 trout types. Implications of the results for future management of Caspian trout populations are discussed.


KEY WORDS: Viral haemorrhagic septicaemia virus · Caspian trout · Susceptibility · Immunity


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Cite this article as: Karami AM, Bani A, Pourkazemi M, Ghasemi M, Kania PW, Buchmann K (2018) Comparative susceptibilities and immune reactions of wild and cultured populations of Caspian trout Salmo trutta caspius to VHSV. Dis Aquat Org 128:187-201. https://doi.org/10.3354/dao03231

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