Inter-Research > DAO > v129 > n1 > p53-62  
DAO
Diseases of Aquatic Organisms

via Mailchimp

DAO 129:53-62 (2018)  -  DOI: https://doi.org/10.3354/dao03225

Ultrastructural and molecular characteristics of Kudoa crenimugilis n. sp. infecting intestinal smooth muscle of fringelip mullet Crenimugil crenilabis in the Red Sea

Abdel-Azeem S. Abdel-Baki1,2,*, Heba M. Abdel-Haleem2, Saleh Al-Quraishy1, Carlos Azevedo3, Lamjed Mansour1,4

1Zoology Department, College of Science, King Saud University, Saudi Arabia, PO Box 2455, Riyadh 11451, Saudi Arabia
2Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt
3Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, Porto 4200-465, Portugal
4Unité de Recherche de Biologie intégrative et Ecologie évolutive et Fonctionnelle des Milieux Aquatiques, Département de Biologie, Faculté des Sciences de Tunis, Université De Tunis El Manar, Tunis 1068, Tunisia
*Corresponding author:

ABSTRACT: This study describes infection of intestinal smooth muscle in fringelip mullets Crenimugil crenilabis with Kudoa crenimugilis n. sp. Of 30 individuals sampled from the Red Sea off Saudi Arabia, 6 (20%) were infected. Ovoid plasmodia (279-412 × 157-295 µm) in the smooth muscle of the intestine were packed with only mature myxospores with 4 valves. Specifically, light and transmission electron microscopy revealed quadrate myxospores with 4 equal, rounded, spore valves uniting at thin delicate suture lines. The mature myxospores were 8 (7-9) µm long, 5.2 (5-6) µm thick and 7.8 (7-8) µm wide. The 4 polar capsules were equal-sized, elliptical to ovoid, and measured 5 (4-5) µm long and 2 (1.5-3) µm wide, possessing 2 filament coils. The sporoplasm was uninucleated and composed of a primary cell enveloping a secondary cell. The parasite had a significant histopathological impact since the developing plasmodia replaced normal muscle tissue and was associated with the myolysis of local muscle fibres and the inflammatory infiltration of lymphocytes and macrophages. The partial sequences of the 18S and 28S rDNA showed that K. crenimugilis n. sp. has the highest level of nucleotide similarity with K. ciliatae (98.46 and 94.11%, respectively) and K. cookii (97.51 and 92.11%, respectively), both of which have previously been reported from the intestines of their host fish. Phylogenetic analysis revealed that K. crenimugilis consistently clustered with these other 2 intestinal Kudoa species in a well-supported subclade, confirming the evaluative association between Kudoa species infecting the same organs.


KEY WORDS: Myxosporea · Kudoa · Histozoic · Intestine · TEM · SSU rRNA · LSU rRNA


Full text in pdf format 
Cite this article as: Abdel-Baki AAS, Abdel-Haleem HM, Al-Quraishy S, Azevedo C, Mansour L (2018) Ultrastructural and molecular characteristics of Kudoa crenimugilis n. sp. infecting intestinal smooth muscle of fringelip mullet Crenimugil crenilabis in the Red Sea. Dis Aquat Org 129:53-62. https://doi.org/10.3354/dao03225

Export citation
Mail this link - Contents Mailing Lists - RSS
Facebook - - linkedIn