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Diseases of Aquatic Organisms

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DAO 129:183-191 (2018)  -  DOI: https://doi.org/10.3354/dao03241

Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults

Sudini Fernando1,2, Pongsopee Attasart3, Salin R. Krishna1, Boonsirm Withyachumnarnkul4,5, Rapeepun Vanichviriyakit6,7,*

1Aquaculture and Aquatic Resources Management, Asian Institute of Technology, Pathum Thani 12120, Thailand
2Department of Animal Science, Uva Wellassa University, Badulla, 90000, Sri Lanka
3Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand
4Department of Anatomy, Faculty of Science, Prince of Songkla University, Hatyai, Songkla 90112, Thailand
5AquaAcademy Farm, Tha Chana, Surat Thani 84170, Thailand
6Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) and
7Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
*Corresponding author:

ABSTRACT: Shrimp infected with Penaeus monodon densovirus (PmoDNV) usually display no specific gross signs, but heavy infections can kill postlarvae and retard juvenile growth. In the present study, samples of hepatopancreas, feces, gonads and hemolymph were isolated from male and female P. monodon subadults chronically infected by PmoDNV. Each sample of hepatopancreas and gonad was divided into 2 parts: one for PmoDNV detection by polymerase chain reaction (PCR), and the other for routine histology and immunohistochemistry. The frequency of positive findings via PCR assays was 92% in the hepatopancreas, 57% in feces, 50% in ovary, 35% in hemolymph and 0% in the testis. Using the densitometric value (DV) of the specific band for PmoDNV relative to that of the β-actin gene as an index of the viral load in the samples, no significant differences were observed among sample types and sexes. Hematoxylin-eosin staining of infected hepatopancreas revealed typical PmoDNV inclusions in the nuclei of infected cells. The ovaries with high DV (>1) contained various types of inclusions along the row of the follicular cells or possibly in the connective tissue cells surrounding the oocytes. Using immunohistochemistry with specific probes to detect PmoDNV proteins, a positive reaction was observed in viral inclusions found in infected hepatopancreas and in ovaries with high DV, specifically in the ovarian capsule, hemolymph, oocytes and nuclear inclusions. These results suggest that the localization of PmoDNV in P. monodon is not confined to the hepatopancreas, but rather that the virus can also occur in the ovary; hence, trans-ovarian, vertical transmission of the virus is highly possible.


KEY WORDS: PmoDNV · Shrimp virus · Hepatopancreas · Ovary · Hemolymph · Aquaculture


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Cite this article as: Fernando S, Attasart P, Krishna SR, Withyachumnarnkul B, Vanichviriyakit R (2018) Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults. Dis Aquat Org 129:183-191. https://doi.org/10.3354/dao03241

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