Inter-Research > DAO > v129 > n3 > p199-205  
DAO
Diseases of Aquatic Organisms

via Mailchimp

DAO 129:199-205 (2018)  -  DOI: https://doi.org/10.3354/dao03244

PCR test to specifically detect the apicomplexan ‘X’ (APX) parasite found in flat oysters Ostrea chilensis in New Zealand

Nguyen Thao Suong1,2,*, Jonathan C. Banks2, Stephen C. Webb2, Andrew Jeffs1, Kevin C. Wakeman3,4, Andrew Fidler1

1Institute of Marine Science, University of Auckland, Auckland 1142, New Zealand
2Cawthron Institute, Nelson 7010, New Zealand
3Office of International Affairs, Hokkaido University, Sapporo 060-0810, Japan
4Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan
*Corresponding author:

ABSTRACT: Described here is a polymerase chain reaction (PCR) test to detect the apicomplexan-X (APX) parasite of a flat oyster species, Ostrea chilensis, endemic to New Zealand. The test primers target sequences in the in situ hybridisation probes identified to bind specifically to APX 18S rRNA and amplify a 723 bp DNA product. The test did not amplify 18S rRNA gene sequences of other apicomplexan species, including Toxoplasma gondii, Neospora caninum, Selenidium spp., Cephaloidophorida spp., Lecudina spp. and Thiriotia sp. Of 73 flat oysters identified by histology to be infected with APX at different severities, 69 (95%) tested PCR-positive. Failure to amplify an internal control indicated the presence of PCR inhibitors in the 4 PCR-negative samples. The high analytical sensitivity, specificity and speed of the PCR test should make it a useful tool for detecting APX.


KEY WORDS: Apicomplexan · Parasite · Ostrea chilensis · New Zealand


Full text in pdf format 
Cite this article as: Suong NT, Banks JC, Webb SC, Jeffs A, Wakeman KC, Fidler A (2018) PCR test to specifically detect the apicomplexan ‘X’ (APX) parasite found in flat oysters Ostrea chilensis in New Zealand. Dis Aquat Org 129:199-205. https://doi.org/10.3354/dao03244

Export citation
Mail this link - Contents Mailing Lists - RSS
Facebook - - linkedIn