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Diseases of Aquatic Organisms

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DAO 130:109-115 (2018)  -  DOI: https://doi.org/10.3354/dao03267

Red seabream iridovirus associated with cultured Florida pompano Trachinotus carolinus mortality in Central America

Adrian Lopez-Porras1, Juan A. Morales1, Gilbert Alvarado2, Samantha A. Koda3, Alvin Camus4, Kuttichantran Subramaniam3, Thomas B. Waltzek3, Esteban Soto5,*

1Servicio de Patologia Veterinaria, Universidad Nacional de Costa Rica, Heredia, Costa Rica
2Laboratory of Experimental and Comparative Pathology (LAPECOM), Biology School, University of Costa Rica, Costa Rica
3Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32611, USA
4Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA
5Department of Medicine and Epidemiology, University of California, School of Veterinary Medicine, Davis, California 95616, USA
*Corresponding author:

ABSTRACT: Mariculture of Florida pompano Trachinotus carolinus in Central America has increased over the last few decades and it is now a highly valued food fish. High feed costs and infectious diseases are significant impediments to the expansion of mariculture. Members of the genus Megalocytivirus (MCV), subfamily Alphairidovirinae, within the family Iridoviridae, are emerging pathogens that negatively impact Asian mariculture. A significant mortality event in Florida pompano fingerlings cultured in Central America occurred in October 2014. Affected fish presented with abdominal distension, darkening of the skin, and periocular hemorrhages. Microscopic lesions included cytomegalic ‘inclusion body-bearing cells’ characterized by basophilic granular cytoplasmic inclusions in multiple organs. Transmission electron microscopy revealed arrays of hexagonal virions (155-180 nm in diameter) with electron-dense cores within the cytoplasm of cytomegalic cells. Pathological findings were suggestive of an MCV infection, and the diagnosis was later confirmed by partial PCR amplification and sequencing of the viral gene encoding the myristylated membrane protein. The viral sequence revealed that the fingerlings were infected with an MCV genotype, red seabream iridovirus (RSIV), previously reported only from epizootics in Asian mariculture. This case underscores the threat RSIV poses to global mariculture, including the production of Florida pompano in Central America.


KEY WORDS: Florida pompano · Trachinotus carolinus · Red seabream iridovirus · RSIV · Megalocytivirus · Inclusion body-bearing cells


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Cite this article as: Lopez-Porras A, Morales JA, Alvarado G, Koda SA and others (2018) Red seabream iridovirus associated with cultured Florida pompano Trachinotus carolinus mortality in Central America. Dis Aquat Org 130:109-115. https://doi.org/10.3354/dao03267

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