DAO 130:95-108 (2018) - DOI: https://doi.org/10.3354/dao03261
Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter Etheostoma fonticola
L. R. Iwanowicz1,*, D. D. Iwanowicz1, C. R. Adams1, T. D. Lewis2, T. M. Brandt3, L. R. Sanders1, R. S. Cornman4
ABSTRACT: The fountain darter Etheostoma fonticola (FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cyprini cell line was refractory at all temperatures evaluated. High throughput sequencing technologies facilitated the complete genome sequencing of this virus utilizing ribosomal RNA-depleted RNA extracted from infected CHSE-214 cells. Conventional PCR primer sets were developed for the detection and confirmation of this virus to assist diagnostic screening methods. Phylogenetic analysis suggests this virus belongs to the Aquareovirus A genus. This research provides requisite initial data critical to support hatchery and refugia biosecurity measures for this endangered species.
KEY WORDS: Virus · Aquareovirus · Etheostoma fonticola · Fountain darter · Endangered
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Full text in pdf format Supplementary material | Cite this article as: Iwanowicz LR, Iwanowicz DD, Adams CR, Lewis TD, Brandt TM, Sanders LR, Cornman RS
(2018) Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter Etheostoma fonticola. Dis Aquat Org 130:95-108. https://doi.org/10.3354/dao03261
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