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Diseases of Aquatic Organisms

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DAO 130:95-108 (2018)  -  DOI: https://doi.org/10.3354/dao03261

Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter Etheostoma fonticola

L. R. Iwanowicz1,*, D. D. Iwanowicz1, C. R. Adams1, T. D. Lewis2, T. M. Brandt3, L. R. Sanders1, R. S. Cornman4

1US Geological Survey, Leetown Science Center, 11649 Leetown Road, Kearneysville, WV 25430, USA
2US Fish and Wildlife Service, Southwestern Native Aquatic Resources and Recovery Center, PO Box 219, Dexter, NM 88230, USA
3US Fish and Wildlife Service, San Marcos Aquatic Resources Center, 500 East McCarty Lane, San Marcos, TX 78666, USA
4US Geological Survey, Fort Collins Science Center, 2150 Centre Avenue Building C, Fort Collins, CO 80526, USA
*Corresponding author:

ABSTRACT: The fountain darter Etheostoma fonticola (FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cyprini cell line was refractory at all temperatures evaluated. High throughput sequencing technologies facilitated the complete genome sequencing of this virus utilizing ribosomal RNA-depleted RNA extracted from infected CHSE-214 cells. Conventional PCR primer sets were developed for the detection and confirmation of this virus to assist diagnostic screening methods. Phylogenetic analysis suggests this virus belongs to the Aquareovirus A genus. This research provides requisite initial data critical to support hatchery and refugia biosecurity measures for this endangered species.


KEY WORDS: Virus · Aquareovirus · Etheostoma fonticola · Fountain darter · Endangered


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Cite this article as: Iwanowicz LR, Iwanowicz DD, Adams CR, Lewis TD, Brandt TM, Sanders LR, Cornman RS (2018) Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter Etheostoma fonticola. Dis Aquat Org 130:95-108. https://doi.org/10.3354/dao03261

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