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Diseases of Aquatic Organisms

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DAO 131:157-166 (2018)  -  DOI: https://doi.org/10.3354/dao03287

Sodium chloride treatment effects on rainbow trout suffering from proliferative kidney disease caused by Tetracapsuloides bryosalmonae

Vladimira Enevova1,*, Miroslava Palikova2, Jana Blahova1, Helena Modra3, Libor Vojtek4, Ivana Papezikova2, Frantisek Tichy5, Jan Mares6, Stanislav Navratil2, Vladimir Vecerek1, Zdenka Svobodova1

1Department of Animal Protection, Welfare and Behaviour, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic
2Department of Ecology and Diseases of Game, Fish and Bees, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic
3Department of Territorial Studies, Mendel University in Brno, Zemedelska 1/1665, 613 00 Brno, Czech Republic
4Department of Experimental Biology, Masaryk University Brno, Kamenice 753/5, 625 00 Brno, Czech Republic
5Department of Anatomy, Histology and Embryology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic
6Department of Zoology, Fisheries, Hydrobiology and Apiculture, Mendel University in Brno, Zemedelska 1/1665, 613 00 Brno, Czech Republic
*Corresponding author:

ABSTRACT: The aim of the present study was to evaluate the effect of a long-term sodium chloride bath on rainbow trout Oncorhynchus mykiss naturally infected by Tetracapsuloides bryosalmonae. A total of 106 infected fish were divided into 2 groups. One group was left untreated and the other was treated with sodium chloride in increasing doses up a concentration of 0.8%. After 14 d, treatment was stopped and for a further 7 d the fish response to the sodium chloride bath was observed. Cumulative mortality was significantly lower in the treated group (19.2%) compared to the untreated group (31.5%) after 21 d. This corresponded to the lower but non-significant parasite intensity in kidney and spleen in the treated group after 14 d of treatment. However, lower prevalence of parasites in both tissues was recorded in the untreated group after 21 d of treatment, but a significant difference was observed only in spleen tissue. Furthermore, significant increases in leukocytes, hemoglobin, haematocrit, ferric reducing ability of plasma, and ceruloplasmin, and significant decreases in alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase activities were noticed in the treated group compared to the untreated group. In contrast, significant decreases in lysozyme concentration in the mucus and phagocyte oxidative burst in the blood were observed in the treated group. Histopathological examination revealed proliferative and reparative changes in parenchymatous tissues in the treated group. The 14- and 21-d salt bath used in rainbow trout with proliferative kidney disease was associated with a reduction in mortality and enhanced the reparative phase in the treated group.


KEY WORDS: PKD · Salt bath · Hematological indices · Biochemical indices · Immunological parameters · Oxidative stress parameters · Histopathology · Immunohistochemistry


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Cite this article as: Enevova V, Palikova M, Blahova J, Modra H and others (2018) Sodium chloride treatment effects on rainbow trout suffering from proliferative kidney disease caused by Tetracapsuloides bryosalmonae. Dis Aquat Org 131:157-166. https://doi.org/10.3354/dao03287

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