Inter-Research > DAO > v132 > n1 > p49-56  
DAO
Diseases of Aquatic Organisms

via Mailchimp

DAO 132:49-56 (2018)  -  DOI: https://doi.org/10.3354/dao03302

Virulent bacteriophage of Edwardsiella ictaluri isolated from kidney and liver of striped catfish Pangasianodon hypophthalmus in Vietnam

Hoang A. Hoang1,*, Mai H. Yen1, Vo T. Ngoan1, Le P. Nga1, Dang T. H. Oanh2

1Department of Biotechnology, Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, Vietnam National University - Ho Chi Minh City (VNU-HCM), 268 Ly Thuong Kiet St., District 10, HCMC, Vietnam
2Department of Aquatic Pathology, College of Aquaculture and Fisheries, Can Tho University, Campus II, 3/2 St., Ninh Kieu District, Can Tho, Vietnam
*Corresponding author:

ABSTRACT: Striped catfish Pangasianodon hypophthalmus farmed in the Mekong Delta, Vietnam, represents an important contribution to Vietnamese aquaculture exports. However, these fish are affected by frequent disease outbreaks across the entire region. One of the most common infections involves white spots in the internal organs, caused by the bacterium Edwardsiella ictaluri. In this study, a virulent phage specific to E. ictaluri, designated MK7, was isolated from striped catfish kidney and liver samples and characterized. Morphological analysis indicates probable placement in the family Myoviridae with a 65 nm icosahedral head and a 147 x 19 nm tail. A double-stranded DNA genome of approximately 34 kb was predicted by restriction fragment analysis following digestion with SmaI. The adsorption affinity (ka) of the MK7 phage was estimated as 1.6 x 10-8 ml CFU-1 min-1, and according to a 1-step growth curve, its latent period and burst size were ~45 min and ~55 phage particles per infected host cell, respectively. Of the 17 bacterial strains tested, MK7 only infected E. ictaluri, although other species of Edwardsiella were not tested. E. ictaluri was also challenged in vitro, in both broth and water from a striped catfish pond and was inactivated by MK7 for 15 h in broth and 51 h in pond water. Thus, initial characterization of phage MK7 indicates its potential utility as a biotherapeutic agent against E. ictaluri infection in striped catfish. This is the first report of a lytic phage specific to an important striped catfish pathogen.


KEY WORDS: Phage therapy · Striped catfish · Characterization · Edwardsiella ictaluri · Mekong Delta · Antibiotic resistance


Full text in pdf format 
Cite this article as: Hoang HA, Yen MH, Ngoan VT, Nga LP, Oanh DTH (2018) Virulent bacteriophage of Edwardsiella ictaluri isolated from kidney and liver of striped catfish Pangasianodon hypophthalmus in Vietnam. Dis Aquat Org 132:49-56. https://doi.org/10.3354/dao03302

Export citation
Mail this link - Contents Mailing Lists - RSS
Facebook - - linkedIn