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Diseases of Aquatic Organisms

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DAO 135:107-119 (2019)  -  DOI: https://doi.org/10.3354/dao03390

Investigating the natural resistance of blackfoot pāua Haliotis iris to abalone viral ganglioneuritis using whole transcriptome analysis

Matthew J. Neave, Serge Corbeil*, Kenneth A. McColl, Mark St. J. Crane

Australian Animal Health Laboratory, Private Bag 24, Geelong, VIC 3220, Australia
*Corresponding author:

ABSTRACT: The natural resistance of New Zealand blackfoot pāua Haliotis iris to infection by haliotid herpesvirus-1 (HaHV-1) and to the disease abalone viral ganglioneuritis was investigated in experimentally challenged p-aua using high throughput RNA-sequencing. HaHV-1-challenged p-aua up-regulated broad classes of genes that contained chitin-binding peritrophin-A domains, which seem to play diverse roles in the pāua immune response. The p-aua also up-regulated vascular adhesion protein-1 (VAP-1), an important adhesion molecule for lymphocytes, and chitotriosidase-1 (CHIT-1), an immunologically important gene in mammalian immune systems. Moreover, several blood coagulation pathways were dysregulated in the p-aua, possibly indicating viral modulation. We also saw several indications that neurological tissues were specifically affected by HaHV-1, including the dysregulation of beta-1,4-N-acetylgalactosaminyltransferase (B4GALNT), GM2 ganglioside, neuroligin-4 and the Notch signalling pathway. This research may support the development of molecular therapeutics useful to control and/or manage viral outbreaks in abalone culture.


KEY WORDS: pāua · Abalone · Haliotid herpesvirus · HaHV-1 · Transcriptome · Peritrophin-A · RNA-Seq


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Cite this article as: Neave MJ, Corbeil S, McColl KA, Crane MSJ (2019) Investigating the natural resistance of blackfoot pāua Haliotis iris to abalone viral ganglioneuritis using whole transcriptome analysis. Dis Aquat Org 135:107-119. https://doi.org/10.3354/dao03390

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