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Diseases of Aquatic Organisms

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DAO 138:121-131 (2020)  -  DOI: https://doi.org/10.3354/dao03449

White spot syndrome virus in decapods from Mississippi Sound, USA, and susceptibility of Palaemonetes pugio and Uca panacea to a Chinese isolate

Muhammad Muhammad1,2, Jeffrey M. Lotz2, Reginald B. Blaylock2,*, Stephen S. Curran2

1Aquaculture Division, Fisheries and Marine Affairs Agency of North Aceh District Lhokseumawe, Aceh Province 24351, Indonesia
2Thad Cochran Marine Aquaculture Center, School of Ocean Science and Engineering, University of Southern Mississippi, Ocean Springs, MS 39564, USA
*Corresponding author:

ABSTRACT: The presence and quantity of white spot syndrome virus (WSSV) was surveyed using TaqMan real-time PCR to assess the extent of the virus in Mississippi Sound, USA. A total of 3577 wild decapods comprising 11 species was collected between November 2012 and August 2015. WSSV was present in 10 of the 11 species. Prevalence ranged from 5.1% in Uca rapax to 38.8% in U. spinicarpa. Viral load ranged from 1.8 to 7.3 log10 copies of WSSV µg-1 total DNA. Two Gulf species, Palaemonetes pugio and U. panacea, were injected with a series of doses of a virulent WSSV isolate from China to determine relative susceptibility and virulence because continuing translocation of highly pathogenic isolates of WSSV poses risk to native species. Survival was 0-65% for P. pugio and 5-60% for U. panacea. Median survival time was lower for P. pugio than U. panacea at all doses. Mean (±SD) lethal load was 9.0 ± 8.9 log copies of WSSV µg-1 total DNA in P. pugio and 8.2 ± 8.3 in U. panacea. Mean viral load in survivors was higher in U. panacea than in P. pugio (5.8 ± 6.1 vs. 3.2 ± 3.0 log copies of WSSV µg-1 total DNA); mean viral load was lower in wild individuals of those species (2.9 ± 3.2 for P. pugio and 4.9 ± 5.0 for U. panacea). U. panacea is potentially more tolerant of WSSV than P. pugio and may serve as an important reservoir host in the community.


KEY WORDS: WSSV · Palaemonetes pugio · Uca panacea · Survey · Susceptibility · Gulf of Mexico


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Cite this article as: Muhammad M, Lotz JM, Blaylock RB, Curran SS (2020) White spot syndrome virus in decapods from Mississippi Sound, USA, and susceptibility of Palaemonetes pugio and Uca panacea to a Chinese isolate. Dis Aquat Org 138:121-131. https://doi.org/10.3354/dao03449

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