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Diseases of Aquatic Organisms

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DAO 139:25-33 (2020)  -  DOI: https://doi.org/10.3354/dao03463

Infectious hematopoietic necrosis virus truncated G protein effect on survival, immune response, and disease resistance in rainbow trout

Xiaojing Hua, Ying Feng, Xin Guan, Yanxue Wang, Ying Zhou, Xuanyu Ren, Dechuan Li, Shuai Gao, Jinshan Huang, Xueting Guan, Wen Shi, Min Liu*

College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China
*Corresponding author:

ABSTRACT: The major antigenic protein of infectious hematopoietic necrosis virus (IHNV) is the surface glycoprotein G, which contains neutralizing epitopes that induce the production of immune neutralizing antibodies. In this study, the IHNV G gene sequence was truncated according to bioinformatics principles and then recombinantly expressed via an E. coli expression system. We then assessed the specific antibody immunoglobin M (IgM) levels of rainbow trout immunized with recombinant truncated G protein (emulsified with Freund’s incomplete adjuvant), and showed that antibody IgM levels of immunized fish were significantly higher than in the control group (p < 0.01). The mRNA expression levels of interferon 1 (IFN1) and interleukin-8 (IL-8) were also up-regulated significantly (p < 0.01) in head kidneys and spleens of rainbow trout immunized with recombinant truncated G protein. Also, after challenge with wild-type IHNV HLJ-09 virus on Day 28, rainbow trout immunized with recombinant truncated G protein showed cumulative survival rates of 60%. These results indicate that the truncated G protein of IHNV expressed by the E. coli prokaryotic expression system can be used as a candidate immunogen for an IHNV subunit vaccine, which lays a theoretical foundation for the study of further potential IHNV subunit vaccines.


EY WORDS: Infectious hematopoietic necrosis virus · G protein · Neutralizing antibody · Immunogenicity · Disease resistance


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Cite this article as: Hua X, Feng Y, Guan X, Wang Y and others (2020) Infectious hematopoietic necrosis virus truncated G protein effect on survival, immune response, and disease resistance in rainbow trout. Dis Aquat Org 139:25-33. https://doi.org/10.3354/dao03463

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