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Diseases of Aquatic Organisms

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DAO 140:1-11 (2020)  -  DOI: https://doi.org/10.3354/dao03488

Isolation and maintenance of Batrachochytrium salamandrivorans cultures

Kristyn A. Robinson1, Kenzie E. Pereira2, Molly C. Bletz3, Edward Davis Carter4, Matthew J. Gray4, Jonah Piovia-Scott5, John M. Romansic5, Douglas C. Woodhams3, Lillian Fritz-Laylin1,*

1Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
2Department of Biology, Duquesne University, Pittsburgh, PA 15282, USA
3Department of Biology, University of Massachusetts, Boston, MA 02125, USA
4Department of Forestry, Wildlife, and Fisheries, University of Tennessee, Knoxville, TN 37996, USA
5School of Biological Sciences, Washington State University, Vancouver, WA 98686, USA
*Corresponding author:

ABSTRACT: Discovered in 2013, the chytrid fungus Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, B. dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging threat, we seek to standardize protocols across the field so that results of laboratory studies are reproducible and comparable. We have collated data and experience from multiple labs to standardize culturing practices of Bsal. Here we outline common culture practices including a medium for standardized Bsal growth, standard culturing protocols, and a method for isolating Bsal from infected tissue.


KEY WORDS: Chytridiomycosis · Amphibian disease · Emerging infectious disease · Life cycle · Management


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Cite this article as: Robinson KA, Pereira KE, Bletz MC, Carter ED and others (2020) Isolation and maintenance of Batrachochytrium salamandrivorans cultures. Dis Aquat Org 140:1-11. https://doi.org/10.3354/dao03488

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