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Diseases of Aquatic Organisms

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DAO 140:119-128 (2020)  -  DOI: https://doi.org/10.3354/dao03500

Simultaneous detection of scale drop disease virus and Flavobacterium columnare from diseased freshwater-reared barramundi Lates calcarifer

Pattarawit Kerddee1,2, Ha Thanh Dong3,4, Putita Chokmangmeepisarn5,6, Channarong Rodkhum6,7, Prapansak Srisapoome8, Nontawith Areechon8, Jorge Del-Pozo9, Pattanapon Kayansamruaj1,2,8,*

1Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
2Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand
3Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand
4Faculty of Science, Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Mahidol University, Bangkok 10400, Thailand
5Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
6The international graduate course of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
7Fish Infectious Diseases Research Unit (FID RU), Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
8Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand
9Royal (Dick) School of Veterinary Studies and The Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian EH25 9RG, UK
*Corresponding author:

ABSTRACT: Freshwater farming of barramundi Lates calcarifer in Thailand is a growing sector in aquaculture, but mortalities due to infectious diseases are still a major threat to this industry. In 2018, an episode of severe mortality in juvenile barramundi was noted in a freshwater earth pond site. Fish presented with severe gill necrosis, as well as severe cutaneous hemorrhages, scale loss, and discoloration at the base of dorsal fin (saddleback lesions). Histopathology revealed extensive necrosis of skeletal muscle and gill filaments, as well as basophilic inclusion bodies and megalocytosis in muscle, gill, liver, and kidney. Scale drop disease virus (SDDV) infection was subsequently confirmed by virus-specific semi-nested PCR. Further, DNA sequences of the viral major capsid protein (MCP) and ATPase genes had a respective homology of 99.85 and 99.92% with sequences of SDDV infecting barramundi in saltwater culture. Gill necrosis and saddleback lesions are not typical lesions associated with scale drop syndrome. Their presence was explained by Flavobacterium columnare isolation from the gill, followed by positive F. columnare-specific PCR. To our knowledge, this is the first report of SDDV-associated mortality in freshwater-farmed barramundi. Furthermore, this mortality presented as a concurrent infection with SDDV and F. columnare, with typical lesions of both infections.


KEY WORDS: Concurrent infection · Flavobacterium columnare · Barramundi · Lates calcarifer · Freshwater aquaculture · Scale drop disease virus


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Cite this article as: Kerddee P, Dong HT, Chokmangmeepisarn P, Rodkhum C and others (2020) Simultaneous detection of scale drop disease virus and Flavobacterium columnare from diseased freshwater-reared barramundi Lates calcarifer. Dis Aquat Org 140:119-128. https://doi.org/10.3354/dao03500

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