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Diseases of Aquatic Organisms

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DAO 140:129-141 (2020)  -  DOI: https://doi.org/10.3354/dao03499

Isolation and characterisation of an ISKNV-genotype megalocytivirus from imported angelfish Pterophyllum scalare

Yasuhiko Kawato1,*, Peter G. Mohr2, Mark St. J. Crane2, Lynette M. Williams2, Matthew J. Neave2, David M. Cummins2, Megan Dearnley2, Sandra Crameri2, Clare Holmes2, John Hoad2, Nicholas J. G. Moody2

1Nansei Main Station, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Minami-Ise, Mie 516-0193, Japan
2CSIRO Australian Animal Health Laboratory, Geelong, VIC 3220, Australia
*Corresponding author:

ABSTRACT: Using cultures of the SKF-9 cell line, megalocytivirus AFIV-16 was isolated from imported angelfish Pterophyllum scalare held in quarantine at the Australian border. The cytopathic effect caused by isolate AFIV-16 presented as cell rounding and enlargement, but complete destruction of the infected cell cultures did not occur. The infected cells demonstrated immunocytochemical reactivity with monoclonal antibody M10, which is used for diagnosis of OIE-listed red sea bream iridoviral disease. Using electron microscopy, the virus particles, consisting of hexagonal nucleocapsids, were observed in the cytoplasm of SKF-9 cells. The replication of AFIV-16 in cultured SKF-9 cells was significantly greater at 28°C incubation than at 22 and 25°C incubation, whereas no difference in growth characteristics was observed for red sea bream iridovirus (RSIV) isolate KagYT-96 across this temperature range. Whole genome sequencing demonstrated that AFIV-16 has a 99.96% similarity to infectious spleen and kidney necrosis virus (ISKNV), the type species in the genus Megalocytivirus. AFIV-16 was classified into ISKNV genotype Clade 1 by phylogenetic analysis of the major capsid protein gene nucleotide sequence. This is the first report of whole genome sequencing of an ISKNV genotype megalocytivirus isolated from ornamental fish.


KEY WORDS: Megalocytivirus · ISKNV · Ornamental fish · Virus isolation and characterisation


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Cite this article as: Kawato Y, Mohr PG, Crane MStJ, Williams LM and others (2020) Isolation and characterisation of an ISKNV-genotype megalocytivirus from imported angelfish Pterophyllum scalare. Dis Aquat Org 140:129-141. https://doi.org/10.3354/dao03499

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