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Diseases of Aquatic Organisms

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DAO 140:219-225 (2020)  -  DOI: https://doi.org/10.3354/dao03507

First detection of infectious haematopoietic necrosis virus in farmed rainbow trout in North Macedonia

Aleksandar Cvetkovikj1,#, Vladimir Radosavljevic2,*,#, Argelia Cuenca3, Biljana Strojmanovska4, Jelena Maksimovic-Zoric2, Iskra Cvetkovikj1, Niels Jørgen Olesen3

1Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia
2Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Serbia
3Danmarks Tekniske Universitet Institut for Akvatiske Ressourcer, Kemitorvet, Building 202, 2800 Kgs. Lyngby, Denmark
4Animal Health and Welfare Department, Food and Veterinary Agency, Treta Makedonska Brigada 20, 1000 Skopje, North Macedonia
*Corresponding author:
#These authors contributed equally to this work

ABSTRACT: Infectious haematopoietic necrosis virus (IHNV) is the causative agent of infectious haematopoietic necrosis (IHN), a disease of salmonids responsible for great economic losses. The disease occurs in most parts of the world where rainbow trout is reared but has not been previously reported in North Macedonia. In this study, 150 pooled samples in total, each consisting of organ mix of 10 freshly killed rainbow trout Oncorhynchus mykiss, were collected from 50 trout farms by the Food and Veterinary Agency of North Macedonia as part of the annual surveillance plan for IHN and viral haemorrhagic septicaemia (VHS) control. Screening of samples was done by cell culture and real-time RT-PCR (qRT-PCR). All 150 tested samples were VHS virus (VHSV) qRT-PCR negative. Two samples from different trout farms were IHNV qRT-PCR positive. On cell culture, 1 IHNV qRT-PCR positive sample caused cytopathic effect after 2 passages on EPC cells. The virus, isolated from an asymptomatic rainbow trout fry, was identified by qRT-PCR and designated as MAKIHNV1. The phylogenetic reconstruction indicates that the isolated virus belongs to the European E genogroup, more specifically within the E-1 clade, and is similar to the German, Italian and Iranian isolates. This study has revealed for the first time the presence of IHNV in rainbow trout in North Macedonia. However, it is not possible to make interpretations about the source of infection from the phylogenetic analysis, and the origin of MAKIHNV1 remains unclear.


KEY WORDS: Infectious haematopoietic necrosis virus · IHNV · Rainbow trout · Phylogeny


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Cite this article as: Cvetkovikj A, Radosavljevic V, Cuenca A, Strojmanovska B, Maksimovic-Zoric J, Cvetkovikj I, Olesen NJ (2020) First detection of infectious haematopoietic necrosis virus in farmed rainbow trout in North Macedonia. Dis Aquat Org 140:219-225. https://doi.org/10.3354/dao03507

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