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Diseases of Aquatic Organisms

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DAO 142:147-160 (2020)  -  DOI: https://doi.org/10.3354/dao03531

Efficacy of nano zinc oxide dietary supplements on growth performance, immunomodulation and disease resistance of African catfish Clarias gariepinus

Heba H. Mahboub1,*, Khalid Shahin2,3, Asmaa W. Zaglool4, Elshimaa M. Roushdy4, Shaimaa A. A. Ahmed1

1Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University, PO Box 44519, Zagazig 4511, Sharkia, Egypt
2Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA
3Aquatic Animal Diseases Laboratory, Department of Aquaculture, National Institute of Oceanography and Fisheries, PO Box 43511, Suez, Egypt
4Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, PO Box 44519, Zagazig 4511, Sharkia, Egypt
*Corresponding author:

ABSTRACT: Zinc (Zn) is an important trace element in fish diets that is required for growth, immunity and antioxidant defense mechanisms. The current study assessed the effects of both organic and nanoparticle zinc oxide (ZnO and ZnO-NPs, respectively) on growth performance, immune response and the antimicrobial effect against Pseudomonas aeruginosa in African catfish Clarias gariepinus. Fish were fed either a control diet or diets supplemented with organic ZnO at concentrations of 20 and 30 mg kg-1 or ZnO-NPs at concentrations of 20 and 30 mg kg-1. After 60 d, a subset of the fish was injected intraperitoneally with 3 × 107 CFU ml-1 of P. aeruginosa. Results showed that body weight gain, feed conversion ratio and specific growth rates were significantly increased in ZnO-NPs20 compared to all other groups. The dietary supplementation with 20 mg kg-1 of ZnO-NPs improved the antioxidant status of fish. Moreover, IgM, lysozyme and nitric oxide showed a significant increase in the fish which received the ZnO-NPs20-supplemented diet. A significant upregulation of growth and stress-related genes was seen in the ZnO-NPs20-supplemented group compared to other groups. However, there was no significant difference in the expression of immune-related genes among ZnO-NPs20, ZnO-NPs30 and ZnO30 groups. These findings highlight the potential use of nano-ZnO for improving growth performance, antioxidant status, immunological status and antibacterial activity against P. aeruginosa in African catfish.


KEY WORDS: Catfish · Zinc oxide nanoparticles · Growth performance · Immunity · Oxidative stress · Pseudomonas aeruginosa · Clarias gariepinus


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Cite this article as: Mahboub HH, Shahin K, Zaglool AW, Roushdy EM, Ahmed SAA (2020) Efficacy of nano zinc oxide dietary supplements on growth performance, immunomodulation and disease resistance of African catfish Clarias gariepinus. Dis Aquat Org 142:147-160. https://doi.org/10.3354/dao03531

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