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Diseases of Aquatic Organisms

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DAO 147:87-95 (2021)  -  DOI: https://doi.org/10.3354/dao03629

First report of a disease caused by Bacillus cereus in cultured loach Paramisgurnus dabryanus

Zhang Luo1, Hanchang Sun2, Shuang Hao1, Youfu Ma3, Zhenguo Zhang3, Shouming Feng3, Xiaohui Bai1,*

1Tianjin Fisheries Research Institute, 442 Jiefangnan Road, Tianjin 300221, PR China
2College of Chongqing University of Arts and Sciences, College of Landscape Architecture and Life Science, Chongqing 402160, PR China
3Prevention and Cure Center for Animal Disease in Tianjin, North of Yixingfu, Tianjin 300221, PR China
*Corresponding author:

ABSTRACT: Bacillus cereus is commonly considered a bacterium pathogenic to mammals, but several studies have suggested that it also induces diseases in fish. In 2017 and 2018, 2 strains of B. cereus, NQ-2017-17 and NQ-2018-8, were isolated from diseased large-scale loach Paramisgurnus dabryanus in Tianjin, China, and were considered to be the pathogens responsible for the disease. These stains were identified as B. cereus based on the results of 16S rDNA gene sequence analysis, Vitek biochemical tests, physiological and biochemical tests, and B. cereus group species-specific PCR. Strains NQ-2017-7 and NQ-2018-8 were found to contain virulence genes (e.g. hblA, hblC, hblD, entFM, and bceT) causing pathological damage to the spleen, kidneys, liver, and gills of loach. The median lethal dose (LD50) of NQ-2017-7 and NQ-2018-8 for loach were 1.0 ×106.64 and 1.0 ×106.49 CFU ml-1, respectively. To our knowledge, this is the first report of loach disease caused by a member of the genus Bacillus.


KEY WORDS: Paramisgurnus dabryanus · Loach · Bacillus cereus · Pathogenic · Isolated


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Cite this article as: Luo Z, Sun H, Hao S, Ma Y, Zhang Z, Feng S, Bai X (2021) First report of a disease caused by Bacillus cereus in cultured loach Paramisgurnus dabryanus. Dis Aquat Org 147:87-95. https://doi.org/10.3354/dao03629

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