Inter-Research > DAO > v149 > p11-23  
DAO
Diseases of Aquatic Organisms

via Mailchimp

DAO 149:11-23 (2022)  -  DOI: https://doi.org/10.3354/dao03655

Enterocytozoon hepatopenaei real-time and Shrimp MultiPathTM PCR assay validation for South-East Asian and Latin American strains of Penaeid shrimp

R. J. Moser1,*, L. Franz1, S. M. Firestone2, M. J. Sellars1

1Genics Pty Ltd., Level 5, Gehrman Building, 60 Research Road, St Lucia, QLD 4067, Australia
2Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, VIC 3010, Australia
*Corresponding author:

ABSTRACT: Enterocytozoon hepatopenaei (EHP) infections are a global challenge for the Penaeid shrimp industry with a sharp rise in prevalence over the last 10 yr. EHP is known to cause sub-optimal growth, large size variation and reduced survival of shrimp. Molecular methods development has mainly focussed on 18S rRNA or spore wall protein 1 (SWP1). Due to the specificity and sensitivity issues with previously designed assays for both targets, new molecular assays are needed by the global shrimp industry and regulators to help manage the risks posed by EHP. This paper describes new real-time PCR (qPCR) methods developed for the novel EHP gene targets polar tube protein 2 (PTP2) and spore wall protein 26 (SWP26), whilst also presenting performance metrics of the new Shrimp MultiPathTM technology EHP assay. qPCR assays PTP2G and SWP26G show high amplification efficiency, a limit of detection (LOD) of between 1 and 4 copies, low assay variation and high diagnostic sensitivity (DSe) and specificity (DSp) compared to imperfect reference assays. Similar performance is seen with Shrimp MultiPathTM EHP showing an LOD of 8 copies, low assay variation and high DSe and DSp. These novel molecular targets for EHP and Shrimp MultiPathTM EHP strengthen global efforts to monitor and mitigate risks of EHP infections and outbreaks. Moreover, this study presents novel data on distribution of EHP in shrimp populations from South-East Asia and Latin America, and how sequence variations need to be considered when monitoring EHP in different geographies.


KEY WORDS: Enterocytozoon hepatopenaei · EHP · Spore wall protein · Polar tube protein · Real-time PCR · Shrimp MultiPathTM


Full text in pdf format
Supplementary material 
Cite this article as: Moser RJ, Franz L, Firestone SM, Sellars MJ (2022) Enterocytozoon hepatopenaei real-time and Shrimp MultiPathTM PCR assay validation for South-East Asian and Latin American strains of Penaeid shrimp. Dis Aquat Org 149:11-23. https://doi.org/10.3354/dao03655

Export citation
Mail this link - Contents Mailing Lists - RSS
Facebook - - linkedIn