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Diseases of Aquatic Organisms

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DAO 151:11-22 (2022)  -  DOI:

Metabolic changes in antennal glands of Caribbean spiny lobsters Panulirus argus infected by Panulirus argus virus 1 (PaV1)

Ioreni Margarita Hernández-Velázquez1, Jesús Alejandro Zamora-Briseño1,2, Gloria Ivonne Hernández-Bolio1, Emanuel Hernández-Nuñez1, Enrique Lozano-Álvarez3, Patricia Briones-Fourzán3, Rossanna Rodríguez-Canul1,*

1Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Carr. Mérida—Progreso, CP 97310 Mérida, Yucatán, México
2Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimic®, El Instituto de Ecología, Carretera antigua a Coatepec 351, El Haya, Xalapa, Veracruz, CP 91070, México
3Unidad Académica de Sistemas Arrecifales, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Puerto Morelos, Quintana Roo 77580, México
*Corresponding author:

ABSTRACT: Panulirus argus virus 1 (PaV1) (Family Mininucleoviridae) causes chronic and systemic infection in wild juvenile spiny lobsters Panulirus argus (Latreille, 1804), ending in death by starvation and metabolic wasting. In marine decapods, the antennal gland is involved in osmoregulation and excretion. In this compact organ, fluid is filtered from the hemolymph, and ions are reabsorbed to produce a hypotonic urine. Although PaV1 is released with the urine in infected individuals, little is known regarding the metabolic effect of PaV1 in the antennal gland. The objective of this study was to perform a comparative evaluation of the metabolic profile of the antennal gland of clinically PaV1-infected lobsters versus those with no clinical signs of infection, using proton nuclear magnetic resonance analysis. Overall, 48 compounds were identified, and the most represented metabolites were those involved in carbohydrate, amino acid, energy, and nucleotide metabolism. Most of the metabolites that were down-regulated in the infected group were essential and non-essential amino acids. Some metabolites involved in the urea cycle and carbohydrate metabolism were also altered. This study represents a first approach to the metabolic evaluation of the antennal gland. We broadly discuss alterations in the content of several proteinogenic and non-proteinogenic amino acids and other key metabolites involved in energetic and nucleotide metabolism.

KEY WORDS: 1H-NMR · PaV1 · Panulirus argus · Antennal gland · Metabolic profile

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Cite this article as: Hernández-Velázquez IM, Zamora-Briseño JA, Hernández-Bolio GI, Hernández-Nuñez E, Lozano-Álvarez E, Briones-Fourzán P, Rodríguez-Canul R (2022) Metabolic changes in antennal glands of Caribbean spiny lobsters Panulirus argus infected by Panulirus argus virus 1 (PaV1). Dis Aquat Org 151:11-22.

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