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Diseases of Aquatic Organisms

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DAO 152:47-60 (2022)  -  DOI:

Invasion of fish parasite Prosorhynchoides ozakii (Trematoda: Bucephalidae) into Lake Kasumigaura and surrounding rivers of eastern Japan

Makito Hayashi1, Yoshihiko Sano1, Takanori Ishikawa2, Tomiji Hagiwara3, Mizuki Sasaki4, Minoru Nakao4, Misako Urabe5, Tsukasa Waki1,*

1Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
2College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
3Global Environmental Forum, 8th Floor Kuramae Intelligent Bldg., 3-17-3 Kuramae, Taito, Tokyo 111-0051, Japan
4Department of Parasitology, Asahikawa Medical University, Midorigaoka-Highashi 2-1, Asahikawa 078-8510, Japan
5Department of Ecosystem Studies, Faculty of Environmental Science, The University of Shiga Prefecture, Hikone 522-8533, Japan
*Corresponding author:

ABSTRACT: In 2019 to 2021, the golden mussel Limnoperna fortunei and several freshwater fishes were sampled from 22 sites of the Tone River system including Lake Kasumigaura, Honshu, Japan, to examine the invasion of bucephalid trematodes. The parasite species identification was performed by morphological observation and DNA barcoding based on the sequences of nuclear 28S rDNA and mitochondrial cytochrome c oxidase subunit 1 (cox1). A total of 1719 mussels were collected from 10 sites, and trematode-infected mussels were detected from 8 sites with prevalences between 0.3 and 42.9%. The sporocysts and cercariae were identified as Prosorhynchoides ozakii, a newly introduced species in the river system. A total of 700 fish individuals belonging to 24 species were collected from 15 sites. Two species of catfishes (Silurus asotus and Ictalurus punctatus) harbored mature or immature adults of Pr. ozakii in the intestine with prevalences between 8.3 and 20% including both host species. The metacercariae of Pr. ozakii were found from the fins and epidermis of 13 fish species from 10 sites (prevalence 4.8-100%). Fishes were heavily infected with metacercariae in fins, which were surrounded by the infiltration of hemocytes and rodlet cells. A population genetic analysis of Pr. ozakii did not show an obvious bottleneck, suggesting the possibility that the parasite was intentionally and repeatedly introduced into the river system.

KEY WORDS: Introduced parasite · Invasive species · Japan · Lake Kasumigaura · Prosorhynchoides ozakii · Tone River · Trematode

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Cite this article as: Hayashi M, Sano Y, Ishikawa T, Hagiwara T and others (2022) Invasion of fish parasite Prosorhynchoides ozakii (Trematoda: Bucephalidae) into Lake Kasumigaura and surrounding rivers of eastern Japan. Dis Aquat Org 152:47-60.

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