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Diseases of Aquatic Organisms

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DAO 156:115-121 (2023)  -  DOI:

Epidemiological cut-off values for non-O1/ non-O139 Vibrio cholerae disc diffusion data generated by standardised methods

Peter Smith1,*, Laëtitia Le Devendec2, Eric Jouy2, Emeline Larvor2, Jean Lesne3, Alexander K. T. Kirschner4,5,6, Carmen Rehm4,5,6, Melanie Leopold5,6, Sonja Pleininger7, Florian Heger7, Claudia Jäckel8, Cornelia Göllner8, Jonas Nekat8, Jens Andre Hammerl8, Sandrine Baron2

1School of Natural Science, University of Galway, Galway H91 TK33, Ireland
2Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit, 22440 Ploufragan, France
3École des Hautes Études en Sante Publique, Laboratoire d’Étude et de Recherche en Environnent et Sante, 35000 Rennes, France
4Institute for Hygiene and Applied Immunology - Water Microbiology, Medical University Vienna, Kinderspitalgasse 15, 1090 Austria
5Division Water Quality & Health, Karl Landsteiner University of Health Sciences, Dr. Karl-Dorrek-Straße 30, 3500 Krems, Austria
6Interuniversity Cooperation Centre Water & Health,
7Institute for Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Austria, Waehringer Strasse 25A, 1094 Vienna, Austria
8Consultant Laboratory for Vibrio spp. in Food, Department Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn Str. 8-10, 1089 Berlin, Germany
*Corresponding author:

ABSTRACT: This work generates the data needed to set epidemiological cut-off values for disc-diffusion zone measurements of Vibrio cholerae. The susceptibility of 147 European isolates of non-O1/non-O139 V. cholerae to 19 antibiotics was established using a standardised disc diffusion method which specified incubation of Mueller Hinton agar plates at 35°C. Epidemiological cut-off values were calculated by analysis of the zone size data with the statistically based normalised resistance interpretation method. Cut-off values for 17 agents were calculated by analysis of the aggregated data from all 4 laboratories participating in this study. The cut-off values calculated were ≥18 mm for amoxicillin/clavulanate, ≥18 mm for amikacin, ≥19 mm for ampicillin, ≥27 mm for cefepime, ≥31 mm for cefotaxime, ≥24 mm for ceftazidime, ≥24 mm for chloramphenicol, ≥31 mm for ciprofloxacin, ≥16 mm for erythromycin, ≥ 27 mm for florfenicol, ≥16 mm for gentamicin, ≥23 mm for imipenem, ≥25 mm for meropenem, ≥29 mm for nalidixic acid, ≥28 mm for norfloxacin, ≥13 mm for streptomycin and ≥23 mm for tetracycline. For the other 2 agents the data from 1 laboratory was excluded from the censored aggregation because the data from that laboratory was considered excessively imprecise. The cut-off values for these 2 agents calculated for the aggregation of the data from 3 laboratories were ≥23 mm for trimethoprim and ≥24 mm for trimethoprim/sulfamethoxazole. These zone size data will be submitted to the Clinical Laboratory Standards Institute (CLSI) and European Committee for Antimicrobial Susceptibility Testing (EUCAST) for their consideration in setting international consensus epidemiological cut-off values for non O1/non-O139 V. cholerae.

KEY WORDS: Vibrio cholerae · Epidemiological cut-off values · Antimicrobial susceptibility · Disc diffusion · Normalized resistance interpretation

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Cite this article as: Smith P, Le Devendec L, Jouy E, Larvor E and others (2023) Epidemiological cut-off values for non-O1/ non-O139 Vibrio cholerae disc diffusion data generated by standardised methods. Dis Aquat Org 156:115-121.

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