Inter-Research > DAO > v160 > p7-12  
DAO
Diseases of Aquatic Organisms

via Mailchimp

DAO 160:7-12 (2024)  -  DOI: https://doi.org/10.3354/dao03817

NOTE
PirA- or PirB-binding nanobodies can protect whiteleg shrimp from the acute hepatopancreatic necrosis disease toxin

Sylvia Cheung1, Slade A. Loutet1, Sarah Zaytsoff1, Filip Van Petegem2, Loc H. Tran3, Hamlet Abnousi1,*

1NovoBind Livestock Therapeutics, Vancouver, BC V6E 0C3, Canada
2University of British Columbia, Department of Biochemistry and Molecular Biology, Vancouver, BC V6T 1Z3, Canada
3ShrimpVet Laboratory, Nong Lam University, Ho Chi Minh City 720371, Vietnam
*Corresponding author:

ABSTRACT: Acute hepatopancreatic necrosis disease (AHPND) is a devastating shrimp disease caused by a binary toxin, PirAB, produced by Vibrio parahaemolyticus and other closely related bacteria. To address AHPND, over 300 unique single-domain antibodies (also known as nanobodies) derived from the VHH domains of Lama glama heavy-chain-only antibodies were raised against either PirA or PirB and characterized. Nanobodies were shortlisted based on their affinities for either PirA or PirB, their relative stability in intestinal fluids, and their ability to reduce PirAB-induced death in brine shrimp Artemia salina. From these data, a subset of nanobodies was tested for their ability to reduce AHPND in whiteleg shrimp Penaeus vannamei, and nanobodies targeting either PirA or PirB provided significant disease protection to whiteleg shrimp. These results show that nanobodies can be a new option for shrimp farmers to reduce or eliminate the impact of AHPND on their operations.


Vibrio parahaemolyticus · Penaeus vannamei · Heavy-chain-only antibodies · Early mortality syndrome


Full text in pdf format
Supplementary Material
Cite this article as: Cheung S, Loutet SA, Zaytsoff S, Van Petegem F, Tran LH, Abnousi H (2024) PirA- or PirB-binding nanobodies can protect whiteleg shrimp from the acute hepatopancreatic necrosis disease toxin. Dis Aquat Org 160:7-12. https://doi.org/10.3354/dao03817

Export citation
Share:    Facebook - - linkedIn

 Previous article