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Diseases of Aquatic Organisms

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DAO 21:131-135 (1995)  -  doi:10.3354/dao021131

Detection of Aeromonas salmonicida in wild Atlantic salmon using a specific DNA probe test

Mooney J, Powell E, Clabby C, Powell R

ABSTRACT: The presence of Aeromonas salmonicida, the etiological agent of furunculosis, was examined using polymerase chain reaction (PCR) and DNA probe methodology in wild Atlantic salmon Salmo salar taken from 3 Irish river systems. Blood samples from 61 individual fish were examined and the presence of pathogen was determined in 87% of the fish examined. However, the level of pathogen was extremely low, calculated at less than 100 A. salmonicida genome equivalents per fish. These data imply a widespread low level infection of A. salmonicida amongst wild salmon in Irish rivers.

KEY WORDS: Aeromonas salmonicida . DNA probe . Atlantic salmon

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