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Diseases of Aquatic Organisms

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DAO 21:201-208 (1995)  -  doi:10.3354/dao021201

Development of monoclonal antibodies to iron-regulated outer membrane proteins and lipopolysaccharide of Aeromonas salmonicida

Neelam B, Thompson KD, Price NC, Tatner MF, Adams A, Ellis AE, Stevens L

Two monoclonal antibodies (MAbs) were obtained against the iron-regulated outer membrane proteins (IROMPs) and 4 MAbs were obtained against the lipopolysaccharide (LPS) of Aeromonas salmonicida, as demonstrated by immunoblotting. MAbs directed against LPS reacted with whole bacteria, both in the enzyme linked immunosorbent assay (ELISA) and by the indirect fluorescent antibody technique (IFAT). Of the 2 MAbs directed against the IROMPs, one did not react with whole bacteria either by IFAT or by ELISA, whereas the other reacted weakly with whole bacteria in both the tests. Some potential applications of the MAbs, including screening of bacterial isolates, are also discussed.

Aeromonas salmonicida . Iron-regulated outer membrane proteins . Lipopolysaccharide . Monoclonal antibodies

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