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Diseases of Aquatic Organisms

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DAO 23:83-92 (1995)  -  doi:10.3354/dao023083

Experimental infection of Australian anurans (Limnodynastes terraereginae and Litoria latopalmata) with Bohle iridovirus

Cullen BR, Owens L, Whittington RJ

Challenge studies with Bohle iridovirus (BIV), a recently isolated Australian iridovirus, were performed with 2 Australian anurans. Juvenile Limnodynastes terraereginae and Litoria latopalmata were highly susceptible to BIV, while larval L. terraereginae were less susceptible. L. terraereginae and L. latopalmata juveniles injected intraperitoneally with 103 TCID50 (50% tissue culture infective dose) ml-1 BIV died 6 to 10 d after injection. BIV could be transmitted by natural routes in L. terraereginae with mortality occurring from Day 14 to 26. Mortality occurred on Days 13 and 33 in L. terraereginae juveniles that were bath-challenged with BIV at a titre of 103 TCID50 ml-1. BIV was retained for at least 40 d in the frogs. Larval L. terraereginae were bath-challenged at 100, 101, 102.5 and 104 TCID50 ml-1 BIV. Low mortality occurred in the 102.5 and 104 TCID50 ml-1 challenges, but BIV was isolated from one tadpole in the 101 TCID50 ml-1 treatment sacrificed 16 d post challenge. Pathological changes were similar for larvae and juveniles of both species, and included renal, hepatic, splenic and pulmonary necroses, with pyknosis of erythrocytes also being observed. Immunoperoxidase staining techniques using cross-reactive anti-epizootic haematopoietic necrosis virus (EHNV) polyclonal antibody indicated the presence of BIV in the kidney, liver, lung and spleen of infected juveniles.

Bohle iridovirus . Anuran . Ranavirus . Amphibian decline

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