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Diseases of Aquatic Organisms

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DAO 33:87-92 (1998)  -  doi:10.3354/dao033087

Siderophore production by Aeromonas salmonicida subsp. salmonicida. Lack of strain specificity

A. I. G. Fernandez1, A. F. Fernández1, M. J. Pérez1, T. P. Nieto1,*, A. E. Ellis2

1Dpto Biología Funcional y Ciencias de la Salud, Facultad de Ciencias, Universidad de Vigo, Lagoas-Marcosende, E-36200 Vigo, Spain
2FRS Marine Laboratory, PO Box 101, Victoria Road, Aberdeen AB11 9DB, Scotland, UK
*Addressee for correspondence. E-mail:

Siderophore production, presence of iron-regulated outer membrane proteins and siderophore specificity was determined among 17 isolates of Aeromonas salmonicida subsp. salmonicida obtained from Spain and Scotland. All grew in the presence of ethylenediamine di(o-hydroxy-phenylacetic acid) (EDDA) and siderophore production was detected using chrome azurol S (CAS) agar, confirming the presence of a high-affinity siderophore iron-uptake mechanism. The Arnow test confirmed that all isolates produced a catechol siderophore. Cross-feeding assays with indicator bacteria showed the absence of anguibactin, enterobactin, 2,3-dihydroxybenzoic acid (DHBA) and the hydroxamate siderophore, aerobactin, in the iron-restricted supernants of a representative isolate which cross fed 15/17 A. salmonicida isolates tested. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed the presence of the same 2 major iron-regulated outer membrane proteins (IROMPs) in all isolates when grown in iron-restricted conditions and siderophore strain specificity as assessed by cross-feeding experiments was not apparent. Thus, with respect to IROMP and siderophore production A. salmonicida appears to be a homogeneous species.

Aeromonas salmonicida subsp. salmonicida · Siderophore · Cross-feeding assay

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