Inter-Research > DAO > v35 > n2 > p155-158  
Diseases of Aquatic Organisms

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DAO 35:155-158 (1999)  -  doi:10.3354/dao035155

Small subunit rRNA gene sequences of Aeromonas salmonicida subsp. smithia and Haemophilus piscium reveal pronounced similarities with A. salmonicida subsp. salmonicida

Jacinta M. Thornton1, Dawn A. Austin2, Brian Austin2, Richard Powell1,*

1Department of Microbiology, National University of Ireland, Galway, Ireland
2Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, UK
*Addressee for correspondence. E-mail:

ABSTRACT: The small subunit ribosomal RNA (SSU rRNA) encoding genes from reference strains of Aeromonas salmonicida subsp. smithia and Haemophilus piscium were amplified by polymerase chain reaction and cloned into Escherichia coli cells. Almost the entire SSU rRNA gene sequence (1505 nucleotides) from both organisms was determined. These DNA sequences were compared with those previously described from A. salmonicida subsp. salmonicida, subsp. achromogenes and subsp. masoucida. This genetic analysis revealed that A. salmonicida subsp. smithia and H. piscium showed 99.4 and 99.6% SSU rRNA gene sequence identity, respectively, with A. salmonicida subsp. salmonicida.

KEY WORDS: Aeromonas salmonicida · Haemophilus piscium · Ribosomal RNA genes

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