Inter-Research > DAO > v35 > n3 > p235-238  
Diseases of Aquatic Organisms

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DAO 35:235-238 (1999)  -  doi:10.3354/dao035235

Crayfish plague epizootics in Germany--classification of two German isolates of the crayfish plague fungus Aphanomyces astaci by random amplification of polymorphic DNA

Birgit Oidtmann1,*, Lage Cerenius2, Ines Schmid1, Rudolf Hoffmann1, Kenneth Söderhäll2

1Institute of Zoology, Fish Biology and Fish Diseases, University of Munich, Kaulbachstr. 37, D-80539 Munich, Germany
2Division of Physiological Mycology, University of Uppsala, Villavägen 6, S-752 36 Uppsala, Sweden

ABSTRACT: Following 2 outbreaks of crayfish plague in southern Germany, the causative agent, the oomycete fungus Aphanomyces astaci, was isolated from the diseased Astacus astacus. The identity of the 2 strains was confirmed using established techniques, such as physiology, spore production and the fact that the isolated strains were highly virulent for A. astacus in infection experiments. The relationship between these German strains and other A. astaci strains was investigated using randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The German strains were found to be closely related to a strain that had been isolated from Pacifastacus leniusculus from Lake Tahoe, USA.

KEY WORDS: Crayfish · Aphanomyces astaci · RAPD-PCR

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