Inter-Research > DAO > v36 > n2 > p107-112  
Diseases of Aquatic Organisms

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DAO 36:107-112 (1999)  -  doi:10.3354/dao036107

Time course tissue distribution of infectious salmon anaemia virus in experimentally infected Atlantic salmon Salmo salar

Espen Rimstad1,*, Knut Falk2, Aase B. Mikalsen1, Ann Teig1

1Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, PO Box 8146, Dep., N-0033 Oslo, Norway
2National Veterinary Institute, PO Box 8156, Dep., N-0033 Oslo, Norway

ABSTRACT: Atlantic salmon Salmo salar L. were injected intraperitoneally with infectious salmon anaemia virus (ISAV)-infective tissue homogenate to clarify the tissue distribution of ISAV in a time course study. Fish were sampled at 11 different intervals between 1 and 40 d post-infection (p.i.) and mid-kidney, head kidney, liver, spleen, intestine, gills, muscle and heart were tested for the presence of ISAV by reverse transcriptase polymerase chain reaction (RT-PCR). The results showed that during a disease outbreak, ISAV is present in most organs. It was possible to detect ISAV at all sampling times in at least 1 of the fish examined. However, for the first 8 d p.i. positive RT-PCR results were predominantly found in samples from the head kidney and mid-kidney. Fish giving positive samples after Day 13 p.i. were RT-PCR positive in most organs. These results indicated that between Days 8 to 13 p.i. considerable replication of the virus occurred, combined with wide tissue dissemination.

KEY WORDS: Infectious salmon anaemia virus (ISAV) · ISAV tissue distribution · RT-PCR detection of ISAV

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