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Diseases of Aquatic Organisms

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DAO 37:81-88 (1999)  -  doi:10.3354/dao037081

Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV

Ellen Lorenzen1,*, Bendix Carstensen2,**, Niels Jørgen Olesen1

1Danish Veterinary Laboratory, EU Reference Laboratory for Fish Diseases, Hangøvej 2, DK-8200 Århus N, Denmark
2Steno Diabetes Center, Niels Steensenvej 2, DK-2820 Gentofte, Denmark
**For the statistical analysis the interested reader can obtain a report with a detailed account by contacting the second author. E-mail:

ABSTRACT: Eleven European National Reference Laboratories participated in an inter-laboratory comparison of the susceptibility of 5 selected cell lines to 3 fish pathogenic viruses. The test included viral hemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV), and the cell lines derived from bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papulosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The results showed that for isolation of VHSV, BF-2 and RTG-2 cells performed equally well and had higher sensitivity compared to the other cell lines. For IHNV, EPC and FHM cells gave the best results, and for IPNV it was BF-2 and CHSE-214 cells. FHM cells showed the largest variability among laboratories, whereas EPC was the cell line showing the smallest variability.

KEY WORDS: VHSV · IHNV · IPNV · CHSE-214 · FHM · EPC · BF-2 · RTG-2 · Cell line susceptibility · Inter-laboratory comparison

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