Inter-Research > DAO > v40 > n3 > p225-228  
Diseases of Aquatic Organisms

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DAO 40:225-228 (2000)  -  doi:10.3354/dao040225

Comparison of infectious haematopoietic necrosis virus (IHNV) isolation on monolayers and in suspended cells

P. Hostnik*, V. Jen<i<

Veterinary Faculty, Gerbiceva 60, 1000 Ljubljana, Slovenia

ABSTRACT: A cell culture virus isolation procedure for infectious haematopoietic necrosis virus (IHNV) in the epithelioma papulosum cyprini cell line (EPC) is described. Ovarian fluid samples were collected from fish and tested for IHNV at 9 farms. The samples were inoculated in parallel on 24 h old EPC cell monolayers and in freshly trypsinized cells. The titre of the initial virus isolation and of first passages were compared using the 2 methods for each sample. Titres were consistently higher in suspended cells and this method also proved more sensitive for isolation of IHN virus from ovarian fluids of infected fish.

KEY WORDS: IHNV · EPC · Susceptibility

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