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Diseases of Aquatic Organisms

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DAO 41:91-104 (2000)  -  doi:10.3354/dao041091

Ultrastructure of white spot syndrome virus development in primary lymphoid organ cell cultures

Chung-Hsiung Wang1, Hsi-Nan Yang2, Chih-Yuan Tang2, Chien-Hsin Lu1, Guang-Hsiung Kou2, Chu-Fang Lo2,*

1Department of Entomology, National Taiwan University, Taipei, Taiwan 106, ROC
2Department of Zoology, National Taiwan University, Taipei, Taiwan 106, ROC
*Corresponding author. E-mail:

ABSTRACT: Primary cell cultures from the lymphoid organ of Penaeus monodon were used to investigate in vitro propagation and morphogenesis of white spot syndrome virus (WSSV). Double-strength Leibovitz¹s L15 supplemented with 20% fetal bovine serum, pH 7.5, with a final osmolarity of 530 ± 5 mOsm kg-1 was identified as the most suitable culture medium. In this medium, the lymphoid cells remained viable for more than 1 wk. Migrating cells were inoculated with WSSV, and the consequent cytopathic effects documented by light and electron microscopy. WSSV appears capable of following 2 alternative assembly sequences, one similar to the morphogenesis of the Oryctes rhinocerus virus and another which is more typical of baculoviral assembly. Possible relationships between WSSV, Oryctes virus, and baculoviruses are discussed.

KEY WORDS: WSSV · In vitro propagation · Lymphoid cells

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