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Diseases of Aquatic Organisms

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DAO 42:77-82 (2000)  -  doi:10.3354/dao042077

White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines

Fe O. Magbanua1, Karlo T. Natividad1, Veronica P. Migo1, Catalino G. Alfafara1, Florian O. de la Peña2, Rolando O. Miranda2, Juan D. Albaladejo2, E. Cesar B. Nadala Jr3, Philip C. Loh4, Lourdes Mahilum-Tapay1,*

1National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines-Los Baños, College, Laguna 4031, Philippines
2Bureau of Fisheries and Aquatic Resources, Department of Agriculture, Quezon City 1001, Philippines
3Deptartment of Haematology, University of Cambridge, EABC Site, Long Road, Cambridge CB2 2PT, United Kingdom
4Virus Laboratory, Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822, USA
*Corresponding author. E-mail:

ABSTRACT: The prevalence and geographic distribution of white spot syndrome virus (WSSV) infection among cultured penaeid shrimp in the Philippines was determined from January to May, 1999, using PCR (polymerase chain reaction) protocol and Western blot assays. A total of 71 samples consisting of 18 post-larvae (PL) and 53 juvenile/adult shrimp samples (56 to 150 days-of-culture, DOC) were screened for WSSV. Of the 71 samples tested, 51 (72%) were found positive for WSSV by PCR: 61% (31/51) after 1-step PCR and 39% (20/51) after 2-step, non-nested PCR. Of the PL and juvenile/adult shrimp samples tested, 50 and 79% were positive for WSSV, respectively. By Western blot, only 6 of the 51 (12%) PCR-positive samples tested positive for WSSV. Of the 20 samples negative for WSSV by PCR, all tested negative for WSSV by Western blot assay. This is the first report of the occurrence of WSSV in the Philippines.

KEY WORDS: White spot syndrome virus · Polymerase chain reaction · Western blot

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