Diseases of Aquatic Organisms
DAO is a hybrid research journal on all aspects of disease phenomena in aquatic organisms.
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DOI: https://doi.org/10.3354/dao044017
copiedRecombinant vaccines against infectious hematopoietic necrosis virus: production by the Caulobacter crescentus S-layer protein secretion system and evaluation in laboratory trials
ABSTRACT: We report the development of an IHNV vaccine produced by a new protein production system based on the bacterium Caulobacter crescentus. The subunit vaccines that were tested contain a 184 amino acid segment of the IHNV glycoprotein in different fusion arrangements with the C. crescentus S-layer protein. Relative percent survival of 26 to 34% was demonstrated in rainbow trout fry for a vaccine that contained the 184 amino acid segment of the IHNV glycoprotein fused to the C-terminal one-quarter of the S-layer protein. Inclusion of the universal mammalian T cell epitopes developed from the measles fusion protein or the tetanus toxin protein did not increase the effectiveness of the IHNV-G/S-layer recombinant protein.
KEYWORDS
Benjamin Simon (Co-author)
- Department of Microbiology and the Center for Salmon Disease Research, 220 Nash Hall, Oregon State University, Corvallis, Oregon 97331-3804, USA
John Nomellini (Co-author)
- Department of Microbiology and Immunology, University of British Columbia, #300-6174 University Blvd., Vancouver, British Columbia V6T 1Z3, Canada
Peter Chiou (Co-author)
- Department of Microbiology and the Center for Salmon Disease Research, 220 Nash Hall, Oregon State University, Corvallis, Oregon 97331-3804, USA
Wade Bingle (Co-author)
- Department of Microbiology and Immunology, University of British Columbia, #300-6174 University Blvd., Vancouver, British Columbia V6T 1Z3, Canada
Julian Thornton (Co-author)
- Microtek/Bayotek International Ltd., 6761 Kirkpatrick Crescent, Saanichton, British Columbia V8M 1Z8, Canada
John Smit (Co-author)
- Department of Microbiology and Immunology, University of British Columbia, #300-6174 University Blvd., Vancouver, British Columbia V6T 1Z3, Canada
Jo-Ann Leong (Corresponding Author)
- Department of Microbiology and the Center for Salmon Disease Research, 220 Nash Hall, Oregon State University, Corvallis, Oregon 97331-3804, USA