Diseases of Aquatic Organisms
DAO is a hybrid research journal on all aspects of disease phenomena in aquatic organisms.
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DOI: https://doi.org/10.3354/dao044035
copiedIdentification of fish-parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method
ABSTRACT: Polymerase chain reaction (PCR) with primers specific for the family Myxobolidae was used to amplify a part of the 18S ribosomal RNA gene of Myxobolus species. The length of the amplified fragments was approximately 1600 base pairs. Six Myxobolus species identified on the basis of morphological features were compared using a combined PCR-RFLP method. The cleavage patterns generated by 2 frequent cutter restriction enzymes (HinfI and MspI) were suitable for the differentiation of the examined Myxobolus species.
KEYWORDS
Edit Eszterbauer (Co-author)
- Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, 1581 Budapest, Hungary
Mária Benkó´ (Co-author)
- Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, 1581 Budapest, Hungary
Ádám Dán (Co-author)
- Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, 1581 Budapest, Hungary
- Central Veterinary Institute, Tábornok u. 2, 1149 Budapest, Hungary
Kálmán Molnár (Co-author)
- Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, 1581 Budapest, Hungary