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Diseases of Aquatic Organisms

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DAO 44:17-27 (2001)  -  doi:10.3354/dao044017

Recombinant vaccines against infectious hematopoietic necrosis virus: production by the Caulobacter crescentus S-layer protein secretion system and evaluation in laboratory trials

Benjamin Simon1, John Nomellini2, Peter Chiou1, Wade Bingle2, Julian Thornton3, John Smit2, Jo-Ann Leong1,*

1Department of Microbiology and the Center for Salmon Disease Research, 220 Nash Hall, Oregon State University, Corvallis, Oregon 97331-3804, USA
2Department of Microbiology and Immunology, University of British Columbia, #300-6174 University Blvd., Vancouver, British Columbia V6T 1Z3, Canada
3Microtek/Bayotek International Ltd., 6761 Kirkpatrick Crescent, Saanichton, British Columbia V8M 1Z8, Canada
*Corresponding author. E-mail:

ABSTRACT: We report the development of an IHNV vaccine produced by a new protein production system based on the bacterium Caulobacter crescentus. The subunit vaccines that were tested contain a 184 amino acid segment of the IHNV glycoprotein in different fusion arrangements with the C. crescentus S-layer protein. Relative percent survival of 26 to 34% was demonstrated in rainbow trout fry for a vaccine that contained the 184 amino acid segment of the IHNV glycoprotein fused to the C-terminal one-quarter of the S-layer protein. Inclusion of the universal mammalian T cell epitopes developed from the measles fusion protein or the tetanus toxin protein did not increase the effectiveness of the IHNV-G/S-layer recombinant protein.

KEY WORDS: Infectious hematopoietic necrosis virus · IHNV · Caulobacter crescentus · Fish virus · Rhabdovirus · Vaccines · S-layer · Type I secretion · T cell epitopes

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