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Diseases of Aquatic Organisms

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DAO 44:35-39 (2001)  -  doi:10.3354/dao044035

Identification of fish-parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method

Edit Eszterbauer1,*, Mária Benkó´1, Ádám Dán1,2, Kálmán Molnár1

1Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, 1581 Budapest, Hungary
2Central Veterinary Institute, Tábornok u. 2, 1149 Budapest, Hungary

ABSTRACT: Polymerase chain reaction (PCR) with primers specific for the family Myxobolidae was used to amplify a part of the 18S ribosomal RNA gene of Myxobolus species. The length of the amplified fragments was approximately 1600 base pairs. Six Myxobolus species identified on the basis of morphological features were compared using a combined PCR-RFLP method. The cleavage patterns generated by 2 frequent cutter restriction enzymes (HinfI and MspI) were suitable for the differentiation of the examined Myxobolus species.

KEY WORDS: Myxobolus spp. · Myxosporea · Fish parasite · PCR · RFLP

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