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Diseases of Aquatic Organisms

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DAO 44:69-74 (2001)  -  doi:10.3354/dao044069

Complete nucleotide sequence of the S10 genome segment of grass carp reovirus (GCRV)*

Tao Qiu, Ren-Hou Lu**, Jing Zhang, Zuo-Yan Zhu

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
*The nucleotide sequence data for GCRV S10 reported in this paper has been deposited in the GenBank data base under the accession number AF236688. **Corresponding author. E-mail:

ABSTRACT: Hemorrhagic disease, caused by the grass carp reovirus (GCRV), is one of the major diseases of grass carp in China. Little is known about the structure and function of the gene segments of this reovirus. The S10 genome segment of GCRV was cloned and the complete nucleotide sequence is reported here. The S10 is 909 nucleotides long and contains a large open reading frame (ORF) encoding a protein of 276 amino acids with a deduced molecular weight of approximately 29.7 kDa. Comparisons of the deduced amino acid sequence of GCRV S10 with those of other reoviruses revealed no significant homologies. However, GCRV S10 shared a putative zinc-finger sequence and a similar distribution of hydrophilic motifs with the outer capsid proteins encoded by Coho salmon aquareovirus (SCSV) S10, striped bass reovirus (SBRV) S10, and mammalian reovirus (MRV) S4. It was predicted that this segment gene encodes an outer capsid protein.

KEY WORDS: Grass carp reovirus (GCRV) · S10 genome segment · Nucleotide sequence

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