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Diseases of Aquatic Organisms

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DAO 44:179-182 (2001)  -  doi:10.3354/dao044179

Genome sequence of a VP2/NS junction region of pillar cell necrosis virus (PCNV) in cultured Japanese eel Anguilla japonica

Nam-Sil Lee1,*, Masato Miyata2, Teruo Miyazaki2

1Department of Aquatic Life Medicine, Pukyong National University, 599-1 Daeyon-3 dong, Nam-gu, Pusan 608-737, Korea
2Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu, Mie 514-8507, Japan

ABSTRACT: Pillar cell necrosis virus (PCNV) is an aquatic birnavirus that was isolated from farmed Japanese eel experiencing mass mortality. In this study, a VP2/NS junction region in genome segment A of PCNV was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and sequenced. The VP2/NS region in PCNV had the highest homology with that of a strain Ab of infectious pancreatic necrosis virus (IPNV). This result revealed that PCNV belongs to birnavirus genogroup II.

KEY WORDS: Genome sequence · VP2/NS junction region · Pillar cell necrosis virus · Birnavirus genogroup II

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