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Diseases of Aquatic Organisms

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DAO 46:101-108 (2001)  -  doi:10.3354/dao046101

Production and characterisation of monoclonal antibodies to salmon pancreas disease virus

D. Todd1,*, V. A. Jewhurst2, M. D. Welsh2, B. J. Borghmans2, J. H. Weston2, H. M. Rowley1, D. P. Mackie1, M. F. McLoughlin1

1Department of Agriculture and Rural Development for Northern Ireland (DARDNI) and
2Department of Veterinary Science, Queen¹s University of Belfast, Veterinary Sciences Division, Stormont, Belfast BT4 3SD, United Kingdom

ABSTRACT: Six mouse monoclonal antibodies (mAbs) specific to salmon pancreas disease virus (SPDV) were produced following immunisation with purified virus preparations. These mAbs and 2 mAbs resulting from an earlier investigation were characterised. None of the mAbs possessed virus neutralising activity but all reacted with 4 geographically different SPDV isolates as determined by indirect immunofluorescence. Three mAbs produced positive immunostaining with Western blots of SPDV proteins. The 4H1 mAb reacted with the 53 kDa structural E1 glycoprotein present in virus-infected cells and in gradient-purified virus. Two mAbs, 5A5 and 7B2, which exhibited unusual immunofluorescence staining of the nuclear margin, reacted with a 35 kDa protein, which is present in gradient-purified virus and which is considered to be the capsid protein. A sandwich ELISA, based on the use of mAb 2D9 for capture and a biotinylated conjugate of mAb 7A2 for detection, detected SPDV antigen in virus-infected Chinook salmon embryo-214 cells and gradient-purified virus. These mAbs may be of use in pathogenesis studies and in diagnostic test development.

KEY WORDS: Monoclonal antibody · Salmon pancreas disease virus · Salmonid alphavirus · Atlantic salmon

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