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Diseases of Aquatic Organisms

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DAO 49:71-76 (2002)  -  doi:10.3354/dao049071

Monoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon

Paisarn Sithigorngul1,*, Sombat Rukpratanporn2, Siwaporn Longyant1, Parin Chaivisuthangkura1, Weerawan Sithigorngul1, Piamsak Menasveta2,3

1Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand
2Marine Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology, Ministry of Science, Technology and Environment, Bangkok 10400, Thailand
3Department of Marine Science, Faculty of Science, Chulalongkor, University, Bangkok 10330, Thailand

ABSTRACT: Monoclonal antibodies specific to 22, 67 and 135 kDa proteins of yellow-head virus (YHV) were produced from a mouse immunized with partially purified YHV isolated from the haemolymph of experimentally YHV-infected Penaeus monodon. Four groups of monoclonal antibodies were identified. One group of antibodies bound only to native protein of YHV while the others were specific to 135, 67 and 22 kDa proteins in both native and denatured forms. All antibodies could be used to detect YHV infection by means of dot blot and immunohistochemistry. However, antibodies specific to the 22 kDa protein gave the best immunohistochemistry results in terms of intensity and sharpness of staining.

KEY WORDS: Capsid · Dot-blot · Envelope · Immunohistochemistry · Matrix · Monoclonal antibodies · Penaeus monodon · Viral infection · YHV

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